FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926590
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10150.049.1077232
TACTCGG10450.045.4480253
GCTACTC23250.042.3113441
CGAGTAG10200.039.646813
TTGGGAG65850.039.3128556
CCGAGTA11250.038.454332
AGTAGCT39600.037.2826925
GGAGGCT63800.037.122549
GTAGCTG39850.036.9024166
GAGTAGC26550.036.4848564
GCTGGGA79800.035.0970959
GCACTTT57550.034.7597851
CTCGGGA14550.034.57755
TCGGGAG15400.034.474386
TAGCTGG44500.034.31627
ACTCGGG14450.033.8435134
GGGAGGC89050.033.506618
CCCGAGT14350.033.4565931
CGGAGGT14850.032.590177
AAAGTGC42200.032.5372124
Produced by FastQC (version 0.11.5)