FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803391
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1550.042.5389861
CCGAGTA13100.031.9650142
CGAGTAG13200.031.3647633
CCCGAGT17450.029.418591
TTGGGAG65300.029.2944986
GCTGGGA80100.028.9840649
GAGTAGC30500.028.6876494
ACTCGCG1503.666719E-828.228293
AGTAGCT44800.028.0359385
GTAGCTG46200.027.4679766
GGAGGCT61700.026.8877479
TAGCTGG50050.026.7626137
GGGAGGC79450.026.560748
CGGAGGT11050.026.3704267
CCCGAAT6750.024.4205271
TGGGAGG85850.023.9784747
GCTACTC26050.023.8647541
CCCAAAG62200.023.775521
GGAGGCC46350.023.7263559
TCGCGTT1401.919661E-523.5207865
Produced by FastQC (version 0.11.5)