FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803391
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT13900.036.173297
CTACTCG11550.034.600672
TACTCGG11700.034.5589183
CCGAGTA13900.033.8246042
TTGGGAG69250.033.588256
CGAGTAG14150.032.8947263
GCTACTC29050.032.390891
CGCCGTA1606.002665E-1132.3130194
CTCGGGA13000.031.8235
GGAGGCT67300.031.4228129
CCCGAGT17600.031.276071
TCGGGAG14450.030.5676426
GCTGGGA83700.030.3752179
GAGTAGC32350.029.6481554
GGGAGGC87750.029.614398
AGTAGCT45650.029.0409075
GGCGGAG21050.029.033085
AGGCGGA21300.028.2534944
GTAGCTG45700.028.0703856
TGGGAGG88800.027.4116947
Produced by FastQC (version 0.11.5)