FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12309932
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG66450.081.4234163
CCGAGTA71500.077.540582
TACTCGG94700.074.95753
CTACTCG94300.073.4378052
GAGTAGC170050.072.052084
AGTAGCT281400.071.271575
GCTACTC220900.070.62551
GCTGGGA684300.069.714989
GTAGCTG289100.068.697446
CCCGAGT90450.065.0506741
TAGCTGG333800.062.7512177
GGAGGCT545500.062.449429
TCGGGAG121300.061.99646
AAGTGCT451450.061.8438535
AAAGTGC443000.061.7598154
CCAAAGT491900.059.4603922
CTCGGGA121200.059.102885
AGTGCTG462500.058.999486
CCCAAAG539700.058.940341
ACTCGGG118050.058.5904434
Produced by FastQC (version 0.11.5)