FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12309932
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG67950.080.006313
CTACTCG98600.075.633752
TACTCGG100600.075.191913
GAGTAGC168050.074.9407044
CCGAGTA73750.074.708292
AGTAGCT277050.073.361275
GCTGGGA682350.072.198959
GCTACTC231750.072.058551
GTAGCTG288650.070.534246
AAAGTGC446100.064.880134
AAGTGCT453200.064.55525
GGAGGCT564550.064.408679
TCGGGAG126750.063.8201186
CCCGAGT87950.063.7976341
CCCAAAG524900.063.3244741
CCAAAGT481850.063.3191532
TAGCTGG332450.063.28137
CTCGGGA122750.062.546985
AGTGCTG466750.062.465556
GTGCTGG470150.061.5397267
Produced by FastQC (version 0.11.5)