FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600962
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA15750.038.801952
CGAGTAG15050.038.1051673
GAGTAGC33800.035.7418444
AGTAGCT54000.034.732795
TACTCGG10450.034.6365783
GTAGCTG54050.034.2658166
CTACTCG10950.033.0572972
CCCGAGT19350.032.1514851
TAGCTGG60750.031.4130547
GCTACTC27850.030.9694841
GCTGGGA95900.030.4806189
TTGGGAG72000.029.836256
GGAGGCT71400.029.0263469
CGGAGGT14350.026.8591527
GGGAGGC92500.026.418838
CTCGGGA12900.026.2363455
TCGGGAG15300.024.5787126
CCCAAAG64350.024.1698231
TTTGGGA74000.024.0750985
GCACTTT64200.023.859231
Produced by FastQC (version 0.11.5)