FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883645
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10350.031.8481122
CGGAGGT7750.031.5315747
CGAGTAG10350.029.571193
AGTAGCT34900.027.1180785
GCTGGGA65850.026.4047749
TTGGGAG54800.026.3334226
GAGTAGC25050.026.3153154
GTAGCTG35500.025.9523816
CCCGAGT14700.025.9518971
TACTCGG8350.025.9398333
AGGCGGA11550.025.6830644
GGAGGCT53400.025.608749
GGGAGGC63550.025.2163858
CCGATAG1151.3585902E-424.5728131
CTACTCG7900.024.4389632
GGAGGTT24700.024.1629948
TAGCTGG42600.023.7177187
CGTCGAG1000.001738149223.5489461
GGCGGAG12850.023.4512025
TCGGGAG11050.023.3964446
Produced by FastQC (version 0.11.5)