FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8084282
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG30700.058.216323
CTACTCG31400.055.9440162
CGAGTAG25350.054.3094253
CCGAGTA28650.050.286192
GCTACTC80850.050.230111
CGGAGGT49000.049.8782967
GGAGGCT190200.049.0418329
GCTGGGA242650.048.5655259
TCGCGTT2900.047.9094735
CCCGAGT39350.047.261961
GAGTAGC70050.046.339544
CTCGGGA39550.044.710375
TCGGGAG42700.044.5179676
AGTAGCT118950.043.9074025
GTAGCTG118450.043.2936
TTGGGAG178100.042.551426
GGCGGAG70950.042.1912775
GCGGAGG58400.042.174366
AGGCGGA71750.041.9849054
CCCAAAG212100.040.7398221
Produced by FastQC (version 0.11.5)