FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7456469
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA31100.059.015192
CGAGTAG27850.058.945123
CTACTCG31550.053.6378972
TACTCGG31800.053.5787543
CCCGAGT41950.052.410721
GAGTAGC69100.050.7539944
GCTGGGA238800.048.747179
GCTACTC82700.048.2005351
AGTAGCT116700.047.15655
GTAGCTG119700.046.2863276
CGGAGGT38900.044.7590147
GGAGGCT188450.044.5466969
CTCGGGA38350.044.4277535
TCGCGTT2950.044.265615
TAGCTGG131750.043.6107337
TCGGGAG41450.043.3553286
TTGGGAG171100.042.521156
CCCAAAG198000.040.2015761
AAAGTGC161100.039.2163624
GTGCTGG160000.038.903287
Produced by FastQC (version 0.11.5)