FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7456469
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG35700.060.0866973
CTACTCG35150.058.7269332
CGAGTAG27250.056.130133
CCGAGTA28450.055.073522
GCTACTC83800.053.2053151
GCTGGGA245000.051.8227049
GAGTAGC72300.051.3412484
AGTAGCT120350.050.2169765
GGAGGCT195550.049.728959
TCGGGAG47100.049.1080176
GTAGCTG122150.048.815256
CTCGGGA44000.048.187245
CCCGAGT38050.047.5574151
TTGGGAG184600.045.571836
TAGCTGG138200.045.1256337
CGGAGGT45600.044.7238737
ACTCGGG50550.042.31244
GTGCTGG163650.041.9833077
AAAGTGC167000.041.5134434
AAGTGCT170350.041.0255665
Produced by FastQC (version 0.11.5)