FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9016320
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG50250.080.340793
TACTCGG67250.076.52593
CCGAGTA53250.076.216342
CTACTCG68600.074.305342
GCTACTC161050.071.3752441
GAGTAGC126300.070.567154
AGTAGCT202100.068.288145
GCTGGGA464750.067.051399
CCCGAGT64700.066.3751751
GTAGCTG211050.065.361696
GGAGGCT387350.063.417299
TCGGGAG89100.061.8851246
ACTCGGG84250.061.543754
CTCGGGA86450.061.1711585
TTGGGAG315150.060.899466
AAGTGCT293700.059.5282865
AAAGTGC287700.059.4920434
TAGCTGG240800.059.295127
CCAAAGT316700.058.5538862
CCCAAAG354500.057.182531
Produced by FastQC (version 0.11.5)