FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9016320
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG50150.076.24923
TACTCGG66700.075.988563
CTACTCG65950.074.7989962
CCGAGTA53650.073.918742
GCTACTC161200.071.2930451
GAGTAGC117500.070.0816964
AGTAGCT188950.068.766945
GCTGGGA465300.068.018639
GTAGCTG200000.064.516536
AAGTGCT295150.064.407465
GGAGGCT397550.064.28199
AAAGTGC294300.063.7170524
TTGGGAG315700.063.2178966
CCCGAGT68050.061.6012841
TCGGGAG88400.061.1287736
CCAAAGT327900.060.8454362
CCCAAAG359600.060.3131141
AGTGCTG313850.059.9538546
ACTCGGG85100.059.482834
TAGCTGG227250.059.4759457
Produced by FastQC (version 0.11.5)