FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3060510
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13200.038.1016583
CCGAGTA14050.036.8032232
TTGGGAG67200.035.5327226
GCTGGGA87800.034.3104639
CCCGAGT18050.033.933631
AGTAGCT46950.033.538475
GTAGCTG46850.033.4093976
GAGTAGC30300.032.2667124
CTACTCG9450.031.835942
TCGCGAA600.004162354431.33286994
TACTCGG10250.031.1830833
GGAGGCT63650.031.010879
TAGCTGG54350.030.7007267
GGGAGGC88350.030.4265378
GCACTTT57250.030.3679241
TTTGGGA69450.029.5762585
CGGAGGT13250.029.0882637
TCGGGAG12700.028.4984366
AAGTGCT46900.028.4629235
CCCAAAG57250.028.3927751
Produced by FastQC (version 0.11.5)