FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3060510
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13200.042.708723
TACTCGG11850.041.2310373
AGTAGCT48700.041.190885
CTACTCG11800.041.0076142
GAGTAGC31100.040.3323024
CCGAGTA14450.039.6644332
GTAGCTG49900.039.5406536
CCCGAGT16950.038.5802461
GCTGGGA91850.038.3087279
TAGCTGG53800.037.0235987
TTGGGAG68500.036.8280566
GCTACTC27600.036.647921
GGAGGCT72300.032.2933549
GCACTTT58000.032.1208531
AAAGTGC48950.031.8630644
CGGAGGT15350.031.8287837
TCGGGAG15850.031.4175036
CCCAAAG62550.031.0629441
TTTGGGA66750.030.8265555
AAGTGCT50950.030.4278965
Produced by FastQC (version 0.11.5)