FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16191847
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG81100.070.7087253
CTACTCG83450.067.656572
CGAGTAG59050.064.5877153
CCGAGTA64200.061.740442
GCTACTC206200.061.6137921
GCTGGGA579250.057.529199
GAGTAGC153500.056.880244
TCGGGAG106100.056.2941556
CCCGAGT78100.056.085121
CTCGGGA104650.055.0570455
GGAGGCT496300.054.3550229
ACTCGGG107200.053.7473874
AGTAGCT258500.052.1658635
GTAGCTG268050.050.3578226
AAAGTGC427000.048.5988924
GTGCTGG424000.048.476647
TAGCTGG291850.048.141397
CGGAGGT114950.047.5757527
AGTGCTG436550.046.989426
AAGTGCT446850.046.8972635
Produced by FastQC (version 0.11.5)