FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10353837
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG46000.067.1971363
CCGAGTA48850.064.630682
TACTCGG59050.062.0777323
GAGTAGC115050.060.7270364
CTACTCG60450.060.0954672
GCTGGGA442200.059.553669
GCTACTC148650.058.7152061
AGTAGCT195850.057.4551625
GTAGCTG197800.056.6605076
CCCGAGT61150.056.2274551
GGAGGCT354800.053.33339
TAGCTGG226400.051.7750937
AAAGTGC304650.051.1123284
TCGGGAG75950.051.0128256
AAGTGCT313750.050.7874765
CCCAAAG360700.050.356331
AGTGCTG312650.050.033286
GTGCTGG310300.049.517147
TTGGGAG296600.049.002166
CCAAAGT336900.048.660452
Produced by FastQC (version 0.11.5)