FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10353837
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG46000.072.352753
CCGAGTA46900.069.837572
TACTCGG61650.069.089013
CTACTCG61900.067.209422
GAGTAGC116650.064.817864
GCTGGGA438250.062.967889
GCTACTC148400.061.3330151
GTAGCTG196650.061.003256
AGTAGCT195150.060.9082035
CCCGAGT58650.058.6744081
GGAGGCT361150.056.3390779
TAGCTGG222050.055.6275377
CTCGGGA76700.054.842335
ACTCGGG76800.054.6852044
AAAGTGC299450.054.6450654
AAGTGCT304650.054.5140765
TCGGGAG80350.054.4778146
AGTGCTG306500.053.485776
GTGCTGG308250.053.0933657
CCCAAAG361250.052.950461
Produced by FastQC (version 0.11.5)