FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12731049
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG54200.060.3654023
CTACTCG55950.058.615292
CCGAGTA42800.058.2095952
CGAGTAG40950.056.5805133
GCTACTC145150.051.66171
GCTGGGA407250.050.904689
GGAGGCT332100.048.9337969
CCCGAGT59200.048.9267431
TCGGGAG72550.048.778656
CTCGGGA71350.047.7273675
CGGAGGT73400.047.302597
GAGTAGC108750.046.8475154
AGTAGCT183950.046.6801765
GTAGCTG189650.044.925116
ACTCGGG75150.043.270444
TAGCTGG211200.043.027087
GTGCTGG285350.042.633227
TTGGGAG279100.041.8189286
CGTTGCG4550.041.164871
CCCAAAG373100.041.021441
Produced by FastQC (version 0.11.5)