FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12731049
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG56000.062.8285523
CTACTCG56450.061.1450232
CGAGTAG41300.058.1951683
CCGAGTA43950.057.1167452
GCTACTC143350.055.160741
GCTGGGA413650.053.3097469
GGAGGCT339500.050.7157259
CTCGGGA70050.050.513095
TCGGGAG72100.050.094856
GAGTAGC110800.050.072094
CCCGAGT58000.048.1309431
AGTAGCT191100.047.024115
ACTCGGG75700.046.8309674
GTAGCTG192550.046.496086
TTGGGAG294500.046.4275446
GTGCTGG297950.045.777927
AAAGTGC303200.044.7874374
CGGAGGT77200.044.5370037
TAGCTGG210800.044.244257
CCCAAAG377200.044.0686041
Produced by FastQC (version 0.11.5)