FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11634635
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG47550.057.939653
CTACTCG47150.057.0195582
CGAGTAG35150.056.4179043
CCGAGTA38150.055.1209532
CCCGAGT50150.051.1041151
GCTACTC118550.050.8174741
GCTGGGA341950.049.9752249
CGGAGGT64450.049.148067
GGAGGCT284850.047.6814089
CTCGGGA58350.047.6717875
GAGTAGC95150.047.4883464
TCGGGAG61950.046.2975776
AGTAGCT165800.043.227215
GTAGCTG166250.042.5894746
ACTCGGG66650.042.533934
TTGGGAG249900.042.0473066
TAGCTGG178800.041.461027
GTGCTGG250700.040.9575427
CCCAAAG321050.040.8469471
GGCGGAG94800.040.2935
Produced by FastQC (version 0.11.5)