FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3236479
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91820.2837033702366059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13550.043.0244872
AGTAGCT51750.042.696655
CGAGTAG13700.040.4943773
GTAGCTG54800.040.2057276
GCTGGGA95450.038.3687939
GAGTAGC34000.038.303124
TAGCTGG60350.038.131117
TACTCGG11300.037.8613663
CCCGAGT16200.036.8806951
TTGGGAG83050.034.561876
CTACTCG11500.034.341142
GCTACTC27600.033.9198881
GGAGGCT75350.033.399589
AAAGTGC46350.033.3717844
CCCAAAG58000.032.9312671
GCACTTT67550.032.732781
CGGAGGT16550.032.0657277
CCAAGTA27400.031.7434752
CAAGTAG26100.031.7033733
CCAAAGT53500.031.4602782
Produced by FastQC (version 0.11.5)