FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2744723
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8500.040.3456572
TACTCGG10100.037.6752783
CTCGGGA10850.035.936955
TTGGGAG54850.034.2566266
CGAGTAG9300.033.8442343
GCTACTC21300.032.474561
TCGGGAG12250.032.2110146
CCGAGTA9800.032.117492
GGAGGCT55550.031.7114669
AGTAGCT36900.029.9181235
CGGAGGT12250.029.143837
GCACTTT50550.029.1359181
GCTGGGA67650.029.0253589
GGGAGGC79000.028.8986998
TTTGGGA55400.028.2375685
CACTTTG52950.028.1245882
GTAGCTG39300.028.0890126
ACTTTGG50650.028.0104683
GAGTAGC24350.027.9744724
GGAGGTT31050.027.6858988
Produced by FastQC (version 0.11.5)