FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3417265
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13850.046.4894143
CCGAGTA14600.044.1012572
AGTAGCT55600.042.5183565
GAGTAGC35800.041.0907974
GTAGCTG57100.040.907566
TTGGGAG84000.040.843876
GCTGGGA103100.040.160639
GCACTTT71600.038.0090261
AAAGTGC54400.037.0630954
CCCGAGT18100.036.938711
TAGCTGG64500.036.505757
ACTTTGG73000.036.1823463
AAGTGCT56900.036.0954485
CACTTTG76150.035.1176642
CCCAAAG69900.035.0939671
CAAAGTG60950.035.0849573
TTTGGGA84150.035.018435
CTACTCG11650.034.694112
CCAAAGT62900.034.595022
TACTCGG11550.034.180673
Produced by FastQC (version 0.11.5)