FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9792253
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG38800.059.576523
CTACTCG40200.056.3926662
CGAGTAG29700.054.0004463
GCTACTC105700.052.3642851
CCGAGTA32200.052.0459632
GGAGGCT239250.050.3020869
CGAATCG3250.050.171207140-144
GCTGGGA298200.049.5442059
CTCGGGA48500.048.1955035
CGGAGGT54400.048.0887227
TCGGGAG51100.047.891896
GAGTAGC80250.047.7367364
CCCGAGT43500.046.0513151
AGTAGCT138850.045.1727945
GTAGCTG143250.045.0889326
ACTCGGG55950.042.1261984
TTGGGAG220800.041.8063286
GTGCTGG208400.041.7392047
TAGCTGG160450.041.6710627
CCCAAAG270450.041.652541
Produced by FastQC (version 0.11.5)