FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9792253
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG41750.069.203513
CTACTCG43600.065.373632
CGAGTAG33550.061.346713
CCGAGTA35700.061.289522
GCTACTC109200.058.1618731
CCCGAGT46600.057.137351
GCTGGGA307950.053.3916179
GGAGGCT262300.052.661289
TCGGGAG55100.052.6559456
CTCGGGA53900.052.512735
GAGTAGC86250.050.1348464
ACTCGGG58800.048.9164
TTGGGAG230900.048.2656176
AGTAGCT142900.047.246325
CGGAGGT60100.046.8724677
GTAGCTG144650.046.621756
CCCAAAG277150.044.9657941
AAAGTGC228000.044.224324
GTGCTGG223250.044.134977
GGGAGGC313100.043.9293638
Produced by FastQC (version 0.11.5)