FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935538
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8950.035.1899833
CTACTCG8900.034.3377652
CCGAGTA11450.030.7967192
CTCGCGT953.7501486E-529.6889554
AGTAGCT35800.029.0186045
CGAGTAG11050.028.5022953
GCTACTC21850.027.8304821
GCTGGGA68050.027.623639
CTCGGGA11950.027.535785
GTAGCTG37850.027.1985346
GGAGGCT49950.027.1901289
TCGCGTT700.00883133126.8614355
GAGTAGC25000.026.512244
ACTCGGG13550.026.3658394
TAAGTCG1458.646093E-725.9351793
TTGGGAG51050.025.782776
CCCGAGT16300.025.44941
CGTCGAG1301.0638902E-525.3826621
TAGCTGG42250.025.031037
TCGGGAG12600.024.996066
Produced by FastQC (version 0.11.5)