FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935538
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT708.646384E-846.9782645
CTACTCG9700.035.8390922
TACTCGG10300.034.663573
CTCGCGT1256.3027983E-933.8243524
GGAGGCT59150.027.4796339
CTCGGGA13450.026.894625
TTGGGAG53450.026.894036
AGTAGCT36350.026.623175
TCGGGAG14150.026.5592446
GCTGGGA66050.026.3870379
CGCCGTA1807.2795956E-926.18691394
GGGAGGC71400.026.186328
GTAGCTG37350.025.6579386
CGGAGGT12650.025.6240717
AGGCGGA16500.024.7703574
CCGAGTA9300.024.751992
TAGCTGG40900.024.6947567
GGAGGTT31100.024.3195258
CGAGTAG9350.024.1171843
GGCGGAG18200.023.4891345
Produced by FastQC (version 0.11.5)