FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183898
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11500.038.5048523
CCGAGTA12450.036.328052
AGTAGCT39850.031.9164125
GAGTAGC26500.031.4639244
ACGATCG753.279968E-431.3282268
CCCGAGT16000.031.2172361
GCTGGGA69250.029.4507929
GTAGCTG42850.029.2866256
TCGCGTT650.0060882428.9884665
TAGCTGG44050.028.5901177
CGGAGGT11750.028.3953677
TACTCGG8850.028.2109743
TTGGGAG59400.028.0108136
CTACTCG8750.027.9985452
GCTACTC21400.027.3033851
GGAGGCT55600.027.2995059
GGGAGGC69750.026.410038
TGGGAGG74800.024.1244097
CGATAGT2650.023.05320742-43
CCCAAAG50450.022.8830261
Produced by FastQC (version 0.11.5)