FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183898
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90540.2843684062743216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT13500.038.262457
CGAGTAG10000.035.2556043
CGCCGTA2050.034.4109594
CTACTCG9850.033.8814162
AGTAGCT38450.033.2489245
CCGAGTA10200.033.1796462
GTAGCTG39600.031.7895096
GAGTAGC24000.031.5316854
TACTCGG10300.031.4904443
TTGGGAG56550.030.8166586
GCGGAGG16850.030.385816
GGAGGCT56200.030.3255529
AGGCGGA20000.029.8473854
GCTGGGA73650.029.5790089
GGAGGTT37800.029.3133498
TAGCTGG45100.028.4249677
GGCGGAG20950.028.2677735
GGAGGCG21150.027.5581952
GAGGTTG46350.026.4380349
GGGAGGC75850.026.2455398
Produced by FastQC (version 0.11.5)