FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8417368
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG30300.057.816373
CTACTCG31800.054.68952
CGAGTAG26850.054.2515753
CCGAGTA30000.051.7673452
GCTACTC83700.049.883021
GCTGGGA254950.049.460319
CGGAGGT42550.047.9109847
CCCGAGT39700.047.8923381
GAGTAGC73100.047.655734
GGAGGCT202900.046.350629
GTAGCTG122500.045.6900636
TCGGGAG40750.044.839516
AGTAGCT126200.044.1258475
CTCGGGA40250.043.6734355
TAGCTGG132900.043.558237
CCCAAAG229350.042.654611
GTGCTGG176200.041.8043637
TTGGGAG188600.041.6766136
AAAGTGC182750.040.406444
GCGGAGG53350.040.2581676
Produced by FastQC (version 0.11.5)