FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9922928
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG63800.083.513963
CCGAGTA69100.080.069182
TACTCGG108050.076.493233
GCTACTC239450.075.846341
GAGTAGC151900.075.075854
CTACTCG109100.074.741352
AGTAGCT246300.073.403915
GCTGGGA656500.071.699079
GTAGCTG260050.070.497426
AAGTGCT414800.067.100025
GGAGGCT557800.066.5041359
AAAGTGC410000.066.242654
TTGGGAG389350.066.010066
CCAAAGT439250.065.634072
CCCGAGT86400.065.4584961
TCGGGAG134100.065.291496
CCCAAAG478400.064.456351
ACTCGGG129950.063.342624
CTCGGGA134700.063.2592475
CAAAGTG445700.062.5994833
Produced by FastQC (version 0.11.5)