FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3411640
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT804.645699E-946.9984665
TACTCGG11500.040.4595533
AGTAGCT54450.039.7911765
CCGAGTA14000.039.2772942
TTGGGAG84900.038.750216
CTACTCG11000.038.0260352
CGAGTAG14350.037.6642763
GTAGCTG57000.037.5163236
GAGTAGC36850.037.1141244
GCACTTT71250.036.8850671
GCTGGGA102800.035.889439
ACTTTGG75950.035.1482963
TAGCTGG62700.034.7803657
GCTACTC27250.034.7400781
CGGAGGT13650.034.4311147
TTTGGGA84450.034.3931965
CCCGAGT17650.034.155961
CACTTTG78850.033.676772
AAAGTGC50850.033.550534
GGAGGCT76150.033.204929
Produced by FastQC (version 0.11.5)