FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8282215
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG42150.068.33123
CCGAGTA44650.067.8203052
CTACTCG54050.066.06672
TACTCGG54350.065.4750753
AGTAGCT172500.064.141395
GAGTAGC108900.063.9393774
GCTACTC126750.062.9231721
GTAGCTG177200.060.9086956
GCTGGGA379350.059.1289679
CCCGAGT56350.057.5773771
TTGGGAG267900.057.530556
GGAGGCT312800.055.48239
TAGCTGG201100.055.1410067
TCGGGAG68900.054.6028866
AAGTGCT225750.053.02775
AAAGTGC227500.051.8061874
CTCGGGA69050.051.536765
AGTGCTG228550.051.4335636
GCACTTT203200.050.8756941
CCAAAGT254250.050.4304962
Produced by FastQC (version 0.11.5)