FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944111
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN81940.27831831068869345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9950.047.2499242
TACTCGG10650.045.0279543
CGAGTAG11550.043.147493
AGTAGCT46750.042.9364245
GTAGCTG49200.040.9621056
CCGAGTA12700.040.720512
GCTGGGA91450.040.4619679
TAGCTGG52450.040.023137
TTGGGAG72300.039.8301776
GAGTAGC30450.039.3703844
GCTACTC26050.038.6460531
CGGAGGT16350.037.627267
CCCGAGT14950.037.4459531
TCGCGTT651.4124581E-436.1606525
CCCAAAG59850.035.4496151
CCAAAGT54550.034.8185582
GGAGGCT70750.034.7783789
AAAGTGC47450.034.7766464
GCACTTT61300.034.3808561
ACTTTGG62200.033.106663
Produced by FastQC (version 0.11.5)