FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10862239
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG51500.062.4570273
CTACTCG51700.060.9809572
CGAGTAG38000.055.457533
CCGAGTA41150.054.249272
GCTACTC134100.052.7364271
GAGTAGC99200.051.3656774
GGAGGCT305200.050.1570789
GCTGGGA362050.050.0998089
TCGGGAG67000.049.1618546
CTCGGGA64500.048.148295
AGTAGCT172100.046.932475
GTAGCTG171300.046.7957276
TTGGGAG264000.045.904316
CCCGAGT57500.045.6951831
TCGCGTT4000.045.6700675
ACTCGGG70800.045.3383264
TAGCTGG195550.043.393757
GGGAGGC359000.042.1140178
AAAGTGC255750.041.8371244
GTGCTGG255800.041.102877
Produced by FastQC (version 0.11.5)