FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288219
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA17150.051.5200652
CGAGTAG16900.050.335523
GAGTAGC37850.045.6945764
AGTAGCT58750.045.278425
CCCGAGT20450.044.6837581
GTAGCTG60100.044.0267456
GCTGGGA106650.043.2759449
TAGCTGG68900.039.9048737
CTACTCG11000.039.7349782
TTGGGAG86250.039.2334146
AAAGTGC51150.039.0504464
TACTCGG11450.037.762873
CCCAAAG67300.037.0238761
AAGTGCT54400.036.9766465
CCAAAGT60600.035.132442
GCTACTC27350.034.616251
GCACTTT69850.034.4584541
CAAAGTG59400.034.4179883
GGAGGCT77600.034.4019059
TTTGGGA84350.033.7095535
Produced by FastQC (version 0.11.5)