FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288219
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT58100.049.1720775
CGAGTAG15600.048.7942123
CCGAGTA15850.048.6167452
GTAGCTG60550.046.9489336
GCTGGGA116300.045.250449
GAGTAGC38650.045.1040424
TACTCGG12750.044.9601253
TAGCTGG69350.043.9042367
TTGGGAG91400.043.0292476
CTACTCG12550.042.3063962
AAAGTGC55700.042.2642974
GCTACTC28950.040.4229281
CCCGAGT20000.039.948081
GCACTTT73200.039.550011
AAGTGCT59100.039.514825
CGGAGGT19500.037.8305787
TTTGGGA85900.037.7986265
GGAGGCT83800.037.792049
CCAAAGT64050.037.7797782
ACTTTGG76800.037.5651443
Produced by FastQC (version 0.11.5)