FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8783009
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG38400.070.3445363
CCGAGTA40900.068.264022
TACTCGG49850.067.181693
CTACTCG50800.064.779552
GAGTAGC102800.062.120884
AGTAGCT168350.062.093225
GCTGGGA376600.061.322019
GCTACTC123900.060.4576381
GTAGCTG173750.060.1271136
CCCGAGT52350.058.1027451
TAGCTGG193900.056.0793957
GGAGGCT301100.055.288159
CTCGGGA60750.054.9053575
TCGGGAG64800.054.539266
AAAGTGC255800.053.3732764
AAGTGCT263750.052.501185
ACTCGGG63950.051.7528344
CCCAAAG305800.051.557041
GTGCTGG260450.051.1686367
AGTGCTG263500.051.045396
Produced by FastQC (version 0.11.5)