FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8783009
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG49150.069.7085953
CGAGTAG39650.069.4224553
CTACTCG50700.065.9152762
CCGAGTA42100.065.5347752
GCTGGGA376950.063.635249
GAGTAGC103450.063.0450064
GCTACTC122000.062.817511
AGTAGCT168850.061.0261425
CCCGAGT49750.060.2897831
GTAGCTG169300.059.357426
GGAGGCT309100.058.166129
AAAGTGC254350.057.4459044
AAGTGCT262550.056.2678155
CCCAAAG309800.055.581391
TAGCTGG189600.055.491077
AGTGCTG262400.054.7562526
TCGGGAG67200.054.729236
CTCGGGA64700.054.355365
GTGCTGG258750.054.348497
CCAAAGT286250.054.1429022
Produced by FastQC (version 0.11.5)