FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11204156
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG58950.077.800773
TACTCGG84450.074.762383
CCGAGTA62950.073.489232
CTACTCG86700.071.75482
GAGTAGC156150.069.872844
GCTACTC197800.067.693361
AGTAGCT253800.067.5987555
GCTGGGA619950.066.65819
GTAGCTG259700.066.464676
CCCGAGT75650.063.7570841
AAAGTGC403400.061.031424
TAGCTGG294450.060.8185587
TCGGGAG113450.060.6249166
GGAGGCT495150.060.540779
ACTCGGG104100.059.999964
AAGTGCT416650.059.820775
CCAAAGT440850.058.9988982
CCCAAAG481050.058.547311
CTCGGGA110900.058.5282175
AGTGCTG423000.058.3476756
Produced by FastQC (version 0.11.5)