FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11204156
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG61850.080.9792563
CCGAGTA65000.078.073822
CTACTCG85500.074.444462
GAGTAGC153200.074.367674
TACTCGG88200.073.81493
GCTACTC205300.071.496911
AGTAGCT253000.071.470855
GCTGGGA616200.068.947019
GTAGCTG263750.068.896696
CCCGAGT79050.065.6339951
AAAGTGC388650.063.1745384
TAGCTGG294650.063.0813567
GGAGGCT504400.062.7751169
AAGTGCT397050.062.7378465
CCAAAGT427250.061.479712
TCGGGAG115850.061.3825046
CCCAAAG464000.061.371681
AGTGCTG406900.060.2490046
TTGGGAG392700.060.0861476
CTCGGGA111550.059.1337475
Produced by FastQC (version 0.11.5)