FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8126093
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG35200.063.037533
CCGAGTA36950.062.2794342
TACTCGG37400.057.4910623
GAGTAGC88700.054.7533764
AGTAGCT143900.054.251935
CTACTCG38750.053.899672
GTAGCTG144300.053.7550136
CGAATCG2450.053.471516140-144
GCTGGGA297900.053.3364269
CCCGAGT49250.052.5046961
GCTACTC94250.050.8864061
TAGCTGG163600.048.941737
GGAGGCT232950.048.834599
CGGAGGT53550.048.2063337
TCGGGAG48350.046.9270946
TTGGGAG209700.046.8562626
CTCGGGA47450.045.4460755
AAAGTGC193700.043.853414
AAGTGCT199000.043.564925
AGTGCTG199050.043.0201456
Produced by FastQC (version 0.11.5)