FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8126093
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG41850.063.1709983
CTACTCG41850.061.9762762
CGAGTAG33850.061.852753
CCGAGTA36700.061.306892
GCTACTC104100.057.4632451
GAGTAGC85050.057.2447934
CCCGAGT45200.055.869131
AGTAGCT140850.055.510265
GCTGGGA305850.054.3357129
GTAGCTG145500.053.0919046
GGAGGCT251600.051.643949
CTCGGGA49950.050.9250835
TTGGGAG223350.049.8648576
TAGCTGG163250.049.6160287
TCGGGAG54450.049.1268276
AAAGTGC195850.046.8782164
CGGAGGT55600.046.3118977
AAGTGCT202450.045.720415
CCCAAAG247500.045.5606881
GTGCTGG197550.045.4305087
Produced by FastQC (version 0.11.5)