FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10156093
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG49550.077.749973
CCGAGTA51850.075.5647052
TACTCGG74750.070.7341163
GCTACTC172850.070.057871
GAGTAGC126300.069.254294
CTACTCG74700.069.202692
GCTGGGA490650.067.395739
AGTAGCT209550.066.316525
GTAGCTG217600.064.314836
CCCGAGT64450.064.2567141
GGAGGCT421400.063.233989
AAAGTGC319650.061.184074
AAGTGCT324100.060.6067965
CCCAAAG384400.059.7487681
TCGGGAG95100.059.5942466
TTGGGAG336000.059.4937936
CCAAAGT351400.059.0863382
CTCGGGA91900.058.8888745
TAGCTGG247000.058.4633067
AGTGCTG330450.058.1137356
Produced by FastQC (version 0.11.5)