FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362961
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95140.282905451475649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13500.045.972942
CGAGTAG13500.043.1873473
CTACTCG10800.043.099632
TACTCGG11300.042.441463
GAGTAGC31100.041.1204224
AGTAGCT51250.040.453985
GTAGCTG50600.040.204466
CCCGAGT17500.039.250961
GCTGGGA89800.038.2258269
TAGCTGG55100.037.9299967
GCTACTC26600.036.081451
TTGGGAG77750.035.531526
GGAGGCT73350.033.6745269
TCGGGAG14850.033.220016
CTCGGGA14950.032.3901255
GGGAGGC103850.031.6077088
GCACTTT64150.031.4627081
AAAGTGC49750.030.9977264
CCCAAAG63000.030.6928161
TTTGGGA77500.030.2095455
Produced by FastQC (version 0.11.5)