FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335709
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1755.4060365E-926.9286771
AGTAGCT42950.024.95755
GTAGCTG45100.024.0804626
GCTGGGA79150.023.512459
CCGAGTA12350.023.2236982
TACTCGG10550.023.1728943
TAGCTGG49350.023.147097
CTACTCG10200.023.0482672
CTCGGGA12700.022.9518325
GGAGGCT61550.022.8295179
GAGTAGC29200.022.702084
ACGTCGA1252.381508E-422.6200891
TCGGGAG13150.022.5239326
GCTACTC26000.021.9313351
CCCGAAT6050.021.0310731
CGAGTAG11750.020.406183
ATCGCGG1405.2027486E-420.1489584
GGGAGGC75650.019.9411268
TTGGGAG58000.019.8594636
CCCGAGT16850.019.8569011
Produced by FastQC (version 0.11.5)