FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335709
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11750.025.9871863
TACTCGG12400.025.3826473
CCGAGTA12700.024.783432
GCTACTC28400.024.6873761
AGTAGCT42450.024.3460645
CTACTCG12400.024.2464732
GGAGGCT66550.024.0711389
GTAGCTG44050.023.248826
GCTGGGA78100.022.6165899
GAGTAGC28100.022.5689454
TTGGGAG58300.021.4340086
TAGCTGG50350.021.272857
CGGAGGT12150.020.4922627
CCCGAGT16850.020.3859021
CGCCGTA3300.019.99821794
TCGGGAG16700.019.6911876
CTCGGGA15300.019.6504445
GGGAGGC78900.019.5888638
GCCGTAT3404.7293724E-1119.41003294
TGGGAGG77000.018.6697
Produced by FastQC (version 0.11.5)