FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4836154
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17350.040.9035573
CCGAGTA17800.040.3975522
GAGTAGC45550.035.2875374
AGTAGCT71650.034.5027055
GTAGCTG72950.033.823436
CCCGAGT24700.033.740451
TTGGGAG98600.033.5090456
CGGAGGT17500.032.4960757
GCTGGGA124750.031.6098929
CTACTCG14650.031.1184352
TACTCGG14850.031.0158163
TAGCTGG80200.030.9416287
GGAGGCT96100.030.9097359
GCACTTT86150.030.8246691
GCTACTC36600.029.7170351
GGGAGGC126950.029.4689038
TCGCGTT1802.382876E-1028.7212815
TTTGGGA104700.027.5616555
TGGGAGG131450.027.3874637
TCGGGAG19550.027.165356
Produced by FastQC (version 0.11.5)