FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10663955
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG36900.066.1482543
CCGAGTA39850.064.685482
TACTCGG43950.059.684243
CTACTCG43200.059.669362
CCCGAGT52100.056.28281
GCTACTC118150.054.380611
GCTGGGA350500.053.502819
GAGTAGC98700.051.8237954
GGAGGCT275050.049.9997759
AGTAGCT164650.049.325395
CGGAGGT61100.048.20887
GTAGCTG167850.048.195346
TCGGGAG57950.047.6915556
TAGCTGG181000.047.06117
CTCGGGA57300.046.33655
GTGCTGG242850.044.446687
CCCAAAG310050.044.1355441
TTGGGAG245550.043.9351846
AAAGTGC247800.043.489344
AGTGCTG251700.043.1458746
Produced by FastQC (version 0.11.5)