FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10663955
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG47700.063.2903483
CGAGTAG38350.062.7731933
CCGAGTA39400.062.0905382
CTACTCG46350.061.0623252
GCTACTC120250.055.5301321
GAGTAGC99400.055.2451254
GCTGGGA354700.053.8225679
CCCGAGT53200.053.46091
AGTAGCT170500.051.2494855
GGAGGCT289600.050.8954359
CGGAGGT68000.050.505717
GTAGCTG172650.050.411486
CTCGGGA57650.050.335395
TCGGGAG60950.049.200236
ACTCGGG61150.048.9439774
TAGCTGG190100.047.6291247
GTGCTGG245800.045.3570067
CCCAAAG312300.045.0765841
TTGGGAG247350.045.0227286
AAAGTGC257650.043.4346354
Produced by FastQC (version 0.11.5)